Order of Draw
According to CLSI guidelines, which of the following represents the correct order of draw for evacuated tubes?
- A. Blue, Yellow, Red/Gold, Green, Lavender, Gray
- B. Yellow, Red/Gold, Blue, Green, Lavender, Gray
- C. Red/Gold, Blue, Yellow, Lavender, Green, Gray
- D. Yellow, Blue, Red/Gold, Green, Lavender, Gray
Answer: D. The CLSI-recommended order of draw for evacuated tubes is: Yellow (SPS/blood culture), Blue (sodium citrate), Red or Gold (serum/SST), Green (heparin), Lavender (EDTA), Gray (fluoride/oxalate). This sequence prevents cross-contamination of additives between tubes.
Venipuncture Procedure
Which vein is the PREFERRED site for routine venipuncture in adults?
- A. Median cubital vein
- B. Basilic vein
- C. Dorsal hand veins
- D. Cephalic vein
Answer: A. The median cubital vein is the preferred site for routine venipuncture because it is typically large, well-anchored, superficial, and located away from major arteries and nerves. The cephalic and basilic veins are acceptable alternatives when the median cubital is not accessible.
Specimen Handling & Labeling
Which of the following information is REQUIRED on a specimen label at the time of collection?
- A. Patient's name, room number, and physician name
- B. Patient's full name, date of birth, date and time of collection, and collector's ID
- C. Patient's full name, date of birth, and the phlebotomist's initials
- D. Patient's insurance information and medical record number only
Answer: B. Per CLSI standards, a properly labeled specimen must include: patient's full name, a unique identifier (date of birth or medical record number), date and time of collection, and the collector's identification. Labels must be applied immediately at the bedside or draw station before leaving the patient.